Thursday, March 22, 2012

Modulated expression of genes associated with NO signal transduction contributes to the cholesterol-lowering effect of electro-acupuncture.




HubMed - Electro Acupuncture





Modulated expression of genes associated with NO signal transduction contributes to the cholesterol-lowering effect of electro-acupuncture.



Biotechnol Lett. 2012 Mar 16;
Li L, Tan GH, Zhang YZ

Electro-acupuncture (EA) at Fenglong acupoint (ST40) can lower the levels of serum cholesterol and triacylglycerols. To study the hepatic genes responsible for the cholesterol-lowering effect of EA, suppression subtractive hybridization combined with the switch mechanism at the 5'-end of RNA template cDNA synthesis and long-distance PCR were employed using hepatic tissues from hypercholesterolemia and EA-treated mice. 68 % of the identified genes are involved in metabolism, immune response, and signal transduction pathways. Real-time PCR and western blot indicate that EA at ST40 induces the expression of nNOS and Mt1, two genes involved in NO signal transduction. EA treatment for hypercholesterolemia thus involves the modulation of several biological pathways and provides a physiological link between NO signal transduction and the cholesterol-lowering effect of EA.








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