HubMed - far infrared therapy
In vivo photoprotective and anti-inflammatory effect of hyperforin is associated with high antioxidant activity in vitro and ex vivo.
Eur J Pharm Biopharm. 2012 Jun; 81(2): 346-50
Meinke MC, Schanzer S, Haag SF, Casetti F, Müller ML, Wölfle U, Kleemann A, Lademann J, Schempp CM
Hyperforin, a major constituent of St. John's Wort (Hypericum perforatum, HP), provides anti-inflammatory, anti-tumor, and anti-bacterial properties. Previous studies have shown anti-oxidative properties of St. John's Wort extracts; however, its free radical scavenging activity in skin cells or skin has not been assessed in detail so far. Therefore, the free radical scavenging activity of hyperforin was tested in the H(2)DCFDA-assay in vitro in HaCaT keratinocytes irradiated with solar simulated radiation. Hyperforin (EC(50) 0.7 μM corresponding to 0.42 μg/ml) was much more effective compared to Trolox (EC(50) 12 μg/ml) and N-acetylcysteine (EC(50) 847 μg/ml) without showing phototoxicity. The radical protection factor of a cream containing 1.5%w/w of a hyperforin-rich HP extract was determined to be 200 × 10(14) radicals/mg, indicating a high radical scavenging activity. The cream was further applied ex vivo on porcine ear skin and significantly reduced radical formation after infrared irradiation. Finally, the UV-protective effect of the HP cream was tested on 20 volunteers in a randomized, double-blind, vehicle-controlled study. HP cream significantly reduced UVB-induced erythema as opposed to the vehicle. Occlusive application of HP cream on non-irradiated test sites did not cause any skin irritation. Taken together, these results demonstrate that hyperforin is a powerful free radical scavenger.
HubMed - Laser Acupuncture
[Blood perfusion in different facial acupoint areas and its changes after acupuncture stimulation of Hegu (LI 4) displayed by laser Doppler imager in healthy volunteers].
Zhen Ci Yan Jiu. 2012 Dec; 37(6): 482-7
Wang SY, Qu XX, Song XJ, Li SY, Ma HM, Zhang D
To observe the blood perfusion levels in different facial acupoint regions and changes after acupuncture stimulation of Hegu (LI 4).Thirty healthy volunteer subjects were equally divided into control group and acupuncture group. Blood perfusion levels of different facial acupoint areas were detected using a Laser Doppler Imager. Acupuncture stimulation was applied to bilateral Hegu (LI 4) by using two filiform needles which were manipulated for a while till "Deqi", followed by keeping them in position for 30 min. The needles were then manipulated once again every 10 min.The blood perfusion levels of facial acupoint regions were from 0.73 to 1.17 PU in healthy volunteers, with relatively higher levels being in Juliao (ST 3), Kouheliao (LI 19), Sibai (ST 2) and Chengqi (ST 1) regions and relatively lower levels in Xiaguan (ST 7), Qianzheng (Ex-HN) and Sizhukong (TE 23) regions, but without significant difference between the isonym acupoint areas of the bilateral face in the control group. Following acupuncture stimulation of bilateral Hegu (LI 4), the blood perfusion volumes were remarkably increased in the acupoint regions on bilateral sides of the face (P < 0.05, P < 0.01).Acupuncture of Hegu (LI 4) can effectively increase blood perfusion volume of the acupoint areas of the bilateral face in healthy volunteers.
HubMed - Low Level Laser therapy
Blue Laser Irradiation Generates Intracellular Reactive Oxygen Species in Various Types of Cells.
Photomed Laser Surg. 2013 Feb 7;
Kushibiki T, Hirasawa T, Okawa S, Ishihara M
Abstract Objective: The purpose of this study was to measure intracellular reactive oxygen species (ROS) production after laser irradiation in various types of cells. Background data: ROS are considered to be the key secondary messengers produced by low-level laser therapy (LLLT). Although various mechanisms for the effects of LLLT have been proposed, and intracellular ROS were indicated as the one of the key factors, direct measurement of intracellular ROS of several types of cells after different wavelength lasers irradiation has not been reported. Materials and methods: Various types of cells were used in this study: mouse preadipocytes (3T3-L1), prechondrocytes (ATDC5), myoblasts (C2C12), mesenchymal stromal cells (KUSA-A1), lung cancer cells (LLC), insulinoma cells (MIN6), fibroblasts (NIH-3T3), human cervix adenocarcinoma cells (HeLa), macrophages differentiated from lymphocytes (THP-1) after treatment with phorbol ester, and rat basophilic leukemia cells (RBL-2H3). Cells were irradiated with a blue laser (wavelength: 405 nm), a red laser (wavelength: 664 nm) or a near infrared laser (wavelength: 808 nm) at 100 mW/cm(2) for 60 or 120 sec. Intracellular ROS levels were measured by fluorometric assay using the intracellular ROS probe, CM-H(2)DCFDA in a flow cytometer. Results: After a blue laser irradiation, intracellular ROS levels were increased in all types of cells. In contrast, intracellular ROS generation was not observed after irradiation with a red laser or near-infrared laser. Conclusions: Potential sources of intracellular ROS were excited by blue laser irradiation, resulting in ROS production within cells. Although the low-level intracellular ROS should be generated after a red or a near-infrared laser irradiation, the only high level intracellular ROS were detected by the ROS probe used in this study. As ROS are considered to be key secondary messengers, the specific functional regulation of cells by laser irradiation will be studied in a future study.
Videos
Healing Skin With Far Infrared
 | healinggrapevine.com Ramona from the Healing GrapeVine has discovered 2 science research papers citing the healing benefits of far infrared therapy on the skin. The research indicates using far infrared on a wound helps it heal quicker and increases the tensile strength which is the measure of how much stress the skin can take before it will break. All of this points to greater evidence that far infrared therapy is GOOD FOR YOU. | Views: 44  1 ratings |
| Time: 06:33 | More in Education |
Google Videos - Acupuncture
This is an excerpt of our monthly show we produce for the Centers for Medicaid and Medicare services. From our "Mind/Body Medicine Show" ...
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